中国对虾淋巴器官中球形病毒的电镜观察     

汝少国  姜明  李永祺  贾翠红  丛日山  王悦 《海洋科学》1999,23(5):44-48

应用光镜和电镜对中国对虾淋巴器官的显微结构和超微结构进行观察,结果表明,淋巴器官的盲管主要由内皮细胞和基质细胞构成,细胞间隙内充满着大量的以颗粒细胞为主的血细胞,  相似文献   

丁字湾增殖中国对虾适宜量的研究   总被引：3，自引：0，他引：3  

信敬福  刘克礼  王四杰  王云中  王树田  刘忠海  宋天林 《海洋科学》1999,23(6):66-67

丁字湾位于山东省海阳、莱阳、即墨三市交界处,面积为１３０ｋｍ２。该海湾局封闭式内湾,不易受风浪影响,地势平坦,潮间带宽阔,水深较浅,最深处１０ｍ。湾内水质肥沃,营养丰富,中国对虾（Ｐｅｎａｅｕｓ　ｃｈｉｎｅｎｓｉｓ喜食的寻氏肌蛤、鸭嘴蛤资源丰厚。该湾春季有亲虾产卵繁殖,６,７月份有幼对虾分布,是进行对虾增殖的优良海湾。但因受饵料和空间等制约,放流数量并非可以无限增加,据１９８８～１９９２年对丁字湾中国对虾放流增殖适宜量的研究表明,随放流数量增加,对虾个体变小,生长减慢,自然死亡率上升。因此,放流…  相似文献   

中国明对虾血蓝蛋白C末端片段在毕赤酵母中的表达及其抗菌活性     

邱楚雯  刘梅  王宝杰  蒋克勇  孙姝娟  孟晓林  骆作勇  王雷 《海洋科学》2013,37(6):1-7

为研究中国明对虾(Fenneropenaeus chinensis)血蓝蛋白C末端(FcHC-C)的抗菌功能,将血蓝蛋白基因FcHC的2个C末端基因片段连接到毕赤酵母表达载体pPIC9K中,构建酵母表达载体pPIC9K/FcHC-C。该载体经SalI酶切后,采用PEG法转化毕赤酵母(Pichia pastoris GS115)。转化子经过PCR鉴定后,阳性克隆通过含有G418的YPD平板筛选,获得高拷贝重组子。重组毕赤酵母利用甲醇诱导表达目的基因。经Tricine-SDS-PAGE和Western blot分析结果表明,利用酵母工程菌成功表达了血蓝蛋白C末端片段(rFcHC-C1和rFcHC-C2)。抑菌活性鉴定实验结果显示,重组蛋白rFcHC-C1和rFcHC-C2作为阴离子抗菌肽具有抗真菌和抗细菌的活性。  相似文献   

铅对不同土壤中青菜生长的影响     

汤莉莉  牛生杰  徐建强  晏培  杨雪贞  谢学俭 《南京气象学院学报》2008,31(1):104-108

采用盆栽试验,研究了南京和北京土壤中Pb污染对青菜生长的影响。结果表明：土壤质地对青菜生长有明显的影响;在土壤Pb含量低的处理水平下,对青菜的生长有一定影响;低含量范围内,青菜中Pb含量随土壤Pb处理含量的增加出现先升后降的变化趋势;在100mg／kg时,青菜的生长受到抑制,植株中Pb的含量高于对照组和200mg／kgPb处理。  相似文献   

复合免疫药物对中国对虾血淋巴抗菌溶菌活力的影响   总被引：3，自引：0，他引：3  

王宜艳  孙虎山  李光友 《海洋科学进展》2004,22(1):69-72

中国对虾口服复合免疫药物“虾康素”后,于第1,3,5．7天分别测定了中国对虾血清中抗菌活力、溶菌活力和酸性磷酸酶(ACP)活力。结果表明,血清中抗菌、溶菌活力和ACP活力在第1,3,5,7天时均为实验组高于对照组,并且均差异极显著。  相似文献   

Peptidomic analysis of Chinese shrimp (Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDI-TOF MS     

王宝杰  刘梅  蒋克勇  张国范  王雷 《中国海洋湖沼学报》2013,31(2):407-415

Peptides in shrimp hemolymph play an important role in the innate immune response.Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection.We used magnetic bead-based purification(ClinProt system) and matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS) to characterize shrimp hemolymph peptides.Shrimp serum and plasma were used as the source of samples for comparative analysis,and it was found that serum was more suitable for shrimp hemolymph peptidomic analysis.To screen potential specific biomarkers in serum of immune-challenged shrimps,we applied magnetic bead-based MALDI-TOF MS to serum samples from 10 immune-challenged and 10 healthy shrimps.The spectra were analyzed using FlexAnalysis 3.0 and ClinProTools 2.1 software.Thirteen peptide peaks significantly different between the two groups were selected as candidate biomarkers of lipopolysaccharide(LPS)-infection.The diagnostic model established by genetic algorithm using five of these peaks was able to discriminate LPS-challenged shrimps from healthy control shrimps with a sensitivity of 90% and a specificity of 100%.Our approach in MALDITOF MS-based peptidomics is a powerful tool for screening bioactive peptides or biomarkers derived from hemolymph,and will help to enable a better understanding of the innate immune response of shrimps.  相似文献   

Construction of a Muscle cDNA Library of Chinese Shrimp Fenneropenaeus chinensis and Sequence Analysis of the Troponin I Gene     

LI Jitao  CHEN Ping  LI Jian  LIU Ping  HE Yuying  WANG Qingyin 《青岛海洋大学学报(英文版)》2010,(1):81-86

  相似文献   

盐肤木对岩溶干旱环境的适应性研究: 叶片解剖结构的启示     

刘彦  赵敏  李忠义  姜建 《中国岩溶》2008,27(3):235-239

通过石蜡切片和扫描电镜观察,研究了岩溶环境下生长的盐肤木叶片结构特征。结果表明,在水分胁迫下,叶片表现了一系列的旱生特征。表皮由一层排列致密的不规则细胞组成。上表皮覆盖一层较厚的角质层,下表皮表面有表皮毛丛生。气孔着生在下表皮,陷入表皮以下。叶肉内栅栏组织发达,海绵组织松散。栅栏组织与海绵组织比约为4∶ 1。部分叶肉细胞中含有胶状结晶物质。叶脉中厚壁组织发达,木质部外纤维数量众多,在气腔周围有单宁结晶分布。这些特征表明,盐肤木采用以保护型为主的多种方式适应岩溶干旱环境。   相似文献   

中国对虾生物能量学研究Ⅰ——温度、体重、盐度和摄食状态对耗氧率和排氨率的影响   总被引：2，自引：0，他引：2  

张硕  董双林  王芳 《中国海洋大学学报(自然科学版)》1998,(2)

实验于１９９６年５月～９月对３种规格养殖中国对虾在不同条件下的耗氧率和排氨率进行了测定。结果表明,温度、体重、盐度和摄食状态对其代谢有显著影响。耗氧率（ＱＯ）和排氨率（ＱＮ）与温度正相关,ｌｎＱＯ＝０．０５８Ｔ－２．８９４;ｌｎＱＮ＝０．０７６Ｔ＋１．１１６;而与体重呈负相关,ＱＯ＝０．５１５Ｗ－０．１２６;ＱＮ＝２５．０５Ｗ－０．１９９。在盐度５～３５范围内,中国对虾（３．０２５ｇ±０．０８８ｇ）的耗氧率和排氨率在盐度２８时最小。中国对虾（３．１３ｇ±０．０７２ｇ）摄食沙蚕和配合饲料,在饱食状态下耗氧率比饥饿状态下分别提高了８３．９％和２９．３％,排泄率增加了１１３．８％和９３．６％,表明摄食时蛋白质代谢增加显著。  相似文献   

Characteristics of Vegetation Carbon,Nitrogen, and C/N Ratio in a Tamarix chinensis Coastal Wetland of China     

Linping Xie  Baodong Wang  Ming Xin  Min Wang  Xiuping He  Qinsheng Wei  Xiaoyong Shi  Xia Sun 《洁净——土壤、空气、水》2019,47(9)

Variations in carbon (C) and nitrogen (N) of the vegetation in a Tamarix chinensis coastal wetland located in Laizhou Bay, China, are analyzed. It is found that T. chinensis accumulates more C and N than the surrounding herbs, and it allocates more C and N in the aboveground parts but less in the roots. Branches store more C, whereas leaves and flowers accumulate more N than other tissues. The C and N contents in the aboveground parts of T. chinensis are mainly influenced by pH, electrical conductivity (EC), water content, and clay content in the top 100 cm of soil as well as the distance from the sea. For the herbs, their C contents vary little in the supratidal zone, but they are higher than those in the intertidal zone. However, N contents in the herbs are higher in the aboveground parts and varied among different communities. The contents of C and N in the herbs are affected by EC, water content as well as the contents of organic C and total nitrogen (TN) in the top 10 cm of soil. The findings confirm that increasing the biomass of T. chinensis is an effective way to increase C sequestration in temperate coastal wetland.  相似文献